Oh goodie the flower is back:-) Having seen one of your experimental shots yesterday I don't think f22 gained you anything and you probably lost some. I always have to go back to Lord V and macro, most of his work is done between f8 and f16, mostly at f11. Since you are using a dedicated macro lens the DOF is a bit different than normal lenses and with this one the resolution falls off pretty substantial at f8 and tanks at f16. This shot doesn't look near as sharp as your other one at f5. So my thought are play around between f5 and f11 I think you will be more than thrilled at the DOF you get at those apertures and sharpness isn't an issue with this lens even at f2.8. Have fun!!